Regulatory

Part:BBa_K4062017

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-16)


lacO, mutation

The lac operator of the Escherichia coli lac operon. The fifth and sixth bases were changed from GT to AC. These changes are shown to greatly reduce the ability of the lac repressor to bind lacO [1].


[1]: Sarah J. Swerdlow and Roel M. Schaaper. "Mutagenesis in the lacI gene target of E. coli: improved analysis for lacI and lacO mutants." Mutat Res. 2014 December 1; 77:79-84


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None